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Taq DNA Ligase (2000u)

von NEB
82,00 €
SKU M0208S

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Try HiFi Taq DNA Ligase, for superior fidelity and thermostability

    Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands. The strands to be ligated need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37° C – 75° C).  

    Product Source

    Purified from an E. coli strain containing the cloned ligase gene from Thermus thermophilus HB8 (1-3).

    Application Features

    • Allele-specific gene detection using Ligase Detection Reaction and Ligase Chain Reaction (4).
    • Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification (5).