Taq DNA Ligase (2000u)
Try HiFi Taq DNA Ligase, for superior fidelity and thermostability
- Isolated from a recombinant source
- Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction
- Taq DNA Ligase is NOT a substitute for T4 DNA Ligase
- Supplied with 10X Reaction Buffer containing NAD+
- Free Thermostable Ligase ReactionTemperature Calculator tool to estimate appropriate incubation temperature
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart or DNA Ligase Selection Chart
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands. The strands to be ligated need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37° C – 75° C).
Purified from an E. coli strain containing the cloned ligase gene from Thermus thermophilus HB8 (1-3).
- Allele-specific gene detection using Ligase Detection Reaction and Ligase Chain Reaction (4).
- Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification (5).