Taq DNA Ligase (2000u)

• Isolated from a recombinant source
• Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction
• Taq DNA Ligase is NOT a substitute for T4 DNA Ligase
• Supplied with 10X Reaction Buffer containing NAD⁺
• Free Thermostable Ligase ReactionTemperature Calculator tool to estimate appropriate incubation temperature
• Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart or DNA Ligase Selection Chart

Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands. The strands to be ligated need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37° C – 75° C).  

Product Source

Purified from an E. coli strain containing the cloned ligase gene from Thermus thermophilus HB8 (1-3).

Application Features

• Allele-specific gene detection using Ligase Detection Reaction and Ligase Chain Reaction (4).
• Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification (5).