T4 Polynucleotide Kinase (2.500 U)
- 5' phosphorylation of DNA/RNA for subsequent ligation
- End labeling DNA or RNA for probes and DNA sequencing
- Removal of 3' phosphoryl groups
Catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´ -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3´-monophosphates. Polynucleotide Kinase also catalyzes the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates (1).
A E. coli strain that carries the cloned T4 Polynucleotide Kinase gene. It is purified by a modification of the method of Richardson (1).
Properties & Usage
10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
0.1 µM ATP
pH 7.4 @ 25°C
65°C for 20 min