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NEBuilder HiFi DNA Assembly Master Mix (50 rxns)

von NEB
- 8%
Ursprünglicher Preis 648,00 €
Aktueller Preis 599,40 €
SKU #E2621L

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NEBuilder® HiFi DNA Assembly Master Mix allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. It has utility for the synthetic biology community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple master-mix format. Visit NEBuilderHifi.com for more information.

  • Also available with competent cells at a discounted price.
  • Enjoy less screening/re-sequencing of constructs, with virtually error-free, high-fidelity assembly
  • Flexible sequence design (scar-less cloning)
  • No PCR clean-up step required
  • Join DNA fragments together more efficiently, even with larger fragments or low DNA inputs
  • Use NEBuilder HiFi in successive rounds of assembly, because it removes 5´ and 3´ end mismatches. (Save time by avoiding time-consuming PCR amplification steps.)
  • Bridge two ds-fragments with a synthetic ss-DNA oligo for simple and fast construction (e.g., linker insertion or gRNA library)
  • Use with lower DNA input requirements
  • Easily adapted for multiple DNA manipulations, including site-directed mutagenesis
  • No licensing fee requirements from NEB for NEBuilder products

NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This methodallows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15–30 bp). It has utility for the synthetic biology community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple master-mix format. The reaction includes different enzymes that work together in the same buffer (see Figure 1):

  • The exonuclease creates single-stranded 3´ overhangs that facilitate the annealing of fragments that share complementarity at one end (the overlap region)
  • The polymerase fills in gaps within each annealed fragment
  • The DNA ligase seals nicks in the assembled DNA 

The end result is a double-stranded fully sealed DNA molecule that can serve as template for PCR, RCA or a variety of other molecular biology applications, including direct transformation of E coli.

Features

Benefits over NEB Gibson Assembly Master Mix and/or In-Fusion HD:

  • Enjoy less screening/re-sequencing of constructs, with virtually error-free, high-fidelity assembly.
  • Join DNA fragments together more efficiently, even with larger fragments or low DNA inputs
  • Use NEBuilder HiFi in successive rounds of assembly, because it removes 5´ and 3´ end mismatches. (Save time by avoiding time-consuming PCR amplification steps.)
  • Bridge two ds-fragments with a synthetic ss-DNA oligo for simple and fast construction (e.g. linker insertion or gRNA library)
  • Use with lower DNA input requirements
  • Switch from other systems easily, because NEBuilder HiFi is compatible with Gibson Assembly®- and In-Fusion- designed fragments
  • No licensing fee requirements from NEB for the NEBuilder products

 

Benefits over Traditional Cloning:

  • Save time with Simple and Fast Seamless Cloning
  • Use one system for both "standard-size" cloning and larger gene assembly products, up to 11 fragments.
  • Move on with your workflow, because DNA can be used immediately for transformation or as template for PCR or RCA.
  • Adapts easily for multiple DNA manipulations, including site-directed mutagenesis.