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Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 1-728) (Active) protein (5µg)

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- 22%
Ursprünglicher Preis 172,50 €
Aktueller Preis 133,69 €
SKU #ABIN2648874

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Application

Functional Studies (Func), SDS-PAGE (SDS)Characteristics

The secreted recombinant human HGF consists of 697 amino acids after cleavage of the signal peptide and has a predicted molecular mass of 79.7 kDa. The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain. As a result of glycosylation, it migrates with the apparent molecular mass of 90, 60 and 34 kDa corresponding to the single chain, α chain and β chain respectively in SDS-PAGE under reducing conditions.

Protein Structure:

A DNA sequence encoding the human HGF (NP_000592.3) precursor (Met 1-Ser 728) was expressed and purified.


Predicted N-Term:

Gln 32 (α chain) & Val 495 (β chain)Purity> 93 % as determined by SDS-PAGEEndotoxin Level< 1.0 EU per μg of the protein as determined by the LAL methodBiological Activity CommentMeasured by its ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation.
The ED50 for this effect is typically 2-10 ng/mL.