Fibroblast Growth Factor 2 (Basic) (FGF2) (AA143-288), (Mature) (Active) protein (100µg)
Functional Studies (Func), SDS-PAGE (SDS)
The recombinant human bFGF is a disulfide-linked homodimeric protein. The reduced monomer consists of 147 amino acids and has a calculated molecular mass of 16.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of bFGF is approximately 17 kDa.
A DNA sequence encoding the mature form of human bFGF (NP_001997.5) (Pro 143-Ser 288) was expressed, with an additional Met at the N-terminus.
Predicted N-Term: MetPurity> 95 % as determined by SDS-PAGEEndotoxin Level< 1.0 EU per mg protein as determined by the LAL method
Biological Activity Comment Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.02-0.1 ng/ml.