DpnI (1000U)

• DpnI cleaves only when its recognition site is methylated.
• Cleavage of mammalian genomic DNA is blocked by overlapping CpG methylation.

Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality.

• Methylation-sensitive restriction enzyme
• Time-Saver™ qualified for digestion in 5-15 minutes
• 100% activity in rCutSmart^™ Buffer (over 215 enzymes are available in the same buffer) simplifying double digests
• Supplied with 1 vial of Gel Loading Dye, Purple (6X)
• Cut Site: GA/TC

Product Source

An E. coli strain that carries the DpnI gene from Diplococcus pneumoniae G41 (S. Lacks).

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of pBR322 DNA (dam methylated) in 1 hour at 37°C in a total reaction volume of 50 µl.

Reaction Conditions

1X rCutSmart™ Buffer
Incubate at 37°C

1X rCutSmart™ Buffer
50 mM Potassium acetate
20 mM Tris-acetate
10 mM Magnesium acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers

NEBuffer™ r1.1: 100%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 75%
rCutSmart™ Buffer: 100%

Diluent Compatibility

• Diluent B

Storage Buffer

10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

80°C for 20 min

Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked by Overlapping

Isoschizomers

MalI