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DNA Polymerase I, Large (Klenow) Fragment

65,00 €
SKU M0210S

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DNA Polymerase I, Large (Klenow) fragment was originally derived as a proteolytic product of E.coli DNA polymerase that retains polymerase and 3’ —> 5’ exonuclease activity

  • Removal of 3’ overhangs or fill-in of 5’ overhangs to form blunt ends
  • Lacks 5’ —> 3’ exonuclease activity
  • Generates probes using random primers
  • Second strand cDNA synthesis

DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3'→ 5' exonuclease activity, but has lost 5'→ 3' exonuclease activity (1). Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.

Highlights

Product Source

An E. coli strain that contains the E. coli polA gene that has had its 5'→3' exonuclease domain removed.

Application Features

  • DNA sequencing by the Sanger dideoxy method (2)
  • Fill-in of 5´ overhangs to form blunt ends (3)
  • Removal of 3´ overhangs to form blunt ends (3)
  • Second strand cDNA synthesis
  • Second strand synthesis in mutagenesis protocols (4)