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BL21(DE3) Competent E. coli (20rxns, 20x50µl)

208,00 €
SKU C2527H

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BL21 (DE3) Competent E. coli is a widely used T7 expression E. coli strain.

  • Ideal for routine T7 expression
  • Protease deficient B strain
  • No dry ice surcharge on competent cell shipments
  • Outgrowth medium included
  • Free of animal products

Chemically competent E. coli cells suitable for transformation and protein expression. 

Highlights

  • Transformation efficiency: 1–5 x 107 cfu/µg pUC19 DNA
  • T7 Expression Strain
  • Deficient in proteases Lon and OmpT
  • Resistant to phage T1 (fhuA2)
  • B Strain
  • Free of animal products

Genotype

fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS
λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5

Reagents Supplied

The following reagents are supplied with this product:

Features

  • Routine T7 expression