ALLin™ Isothermal DNA Amplification Kit (500 reactions of 25 µl)

ALLin™ Isothermal DNA Amplification Kit enables detection of as little as 5 target DNA molecules in a short time of 20 minutes even without the use of a thermal cycler. The Kit includes a 2X master mix with optimized high-performance buffer, dNTPs and a recombinant Bst Polymerase large fragment having strong 5’ - 3’ strand displacement activity and efficient 5’-3’ polymerase activity working at 55-70°C. The Polymerase has neither 5’ - 3’ nor 3’ - 5’ exonuclease activity and retains only minor reverse transcription activity. The kit includes PCR water; only templates and primers have to be supplied by the user. Quantitative Fluorescent Dye, 20X is included for an optional use, for a real-time detection in FAM channel on any qPCR cycler. ALLin™ Isothermal DNA Amplification Kit is a tool of choice for such applications like LAMP, WGA, RAM with an additional advantage of higher temperature reactions, what makes amplification of complex and GC-rich templates more efficient. Technical characteristics of Bst DNA Polymerase large fragment: Strong 5’ - 3’ strand displacement activity. 5’-3’ polymerase activity. No 5’ - 3’ exonuclease activity. No 3’ - 5’ exonuclease (proofreading) activity. Minor reverse transcriptase activity (for RNA, use ALLin™ Isothermal 1Step RNA Amplification Kit (#IRK0101) which includes RTase). Optimal amplification temperature is 65°C. Working temperature range is 55-70°C. Optimal reaction time is 20 minutes, if needed, the reaction can be performed 30 to 60 minutes. The enzyme is inactivated in 10 minutes at 80°C. Quantitative Fluorescent Dye has the excitation max. at 482 nm and emission max. at 512 nm. The use of this product in certain applications may be covered by patents. The user has to analyse all applicable Limited Use Label Licenses and may need licensing for certain cases.

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Keywords:Isothermal DNA Amplification Kit, Bst Polymerase, Bst DNA polymerase